Case of nucleic acid synthesis-2

Sequence: DNA 20mer

Device: STS

Synthesis column: 6.3ml synthesis column

Solid-phase synthesis vector information: unylinker

Synthesis Chromatogram

Crude purity: 88.03%

Crude HPLC Chromatogram

Purification

Sample: Dilute 150μ of crude product to 1 ml

Flow rate: 1ml/min

Column: NanoQ 30L 4.7ml

Buffer A：10 mM NaOH

Buffer B：10 mM NaOH ＋ 2 M NaC

Gradient: 0-40B 20CV

Purification chromatogram

HPLC chromatogram after one-step anion purification

After one-step anion purification and then detection by HPLC, the purity of the target product is as high as 99.14%